Next generation or NGS sequencing is considered to be an important technology for the determination of the RNA or DNA sequence. So, this technology of NGS sequencing is mainly used for studying variation that is linked with several biological phenomena or disease. As a result, the throughput, speed, and accuracy of NGS have hugely revolutionized genetic analysis. Also, it contributes to enabling new applications in forensic, agricultural, environmental science, reproductive health, and clinical and genomic research.